Bioline tetro cdna synthesis kit
WebSensiFAST™ cDNA Synthesis Kit Bioline To complement the SensiFAST™ Probe and SYBR® kits, Bioline has developed the SensiFAST cDNA Synthesis Kit. Efficient – high-target affinity, coupled with a novel TransAmp buffer system for improved yield of full-length cDNA Unbiased – optimized mix of random hexamers… Related Products: Cdna … WebAdditionally, the ISOLATE II RNA Mini Kit can be used to purify samples for PCR and RT-PCR amplification using the Tetro cDNA Synthesis Kit and any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase. Applications RT-qPCR End-point RT-PCR Northern, dot and slot blotting Array analysis Poly A+ RNA selection RNA-Seq
Bioline tetro cdna synthesis kit
Did you know?
WebThe double-stranded cDNA was synthesized using a Tetro cDNA Synthesis Kit (Bioline, London, UK). ... Quantitative real-time PCR was performed using the SensiFAST™ SYBR ® No-ROX kit (Bioline, London, UK) in a qTOWER3 G real-time thermal cycler (Analytik Jena, Jena, Germany) with beta actin (β-actin) as the housekeeping gene. cDNA was diluted ... WebThe polymerase (catalog no. BIO-21105, Bioline, London, UK) was RNA was then converted into the first-strand cDNA using an used for the second-round amplification. The cycling conditions oligo-dT primer and a Tetro cDNA synthesis kit (catalog no. BIO- were initial denaturation at 95°C for 3 min, followed by 40 cycles 65043, Bioline, London ...
WebSnippet: RT-PCR analysis of selected genes RNA extraction was performed using a Qiagen RNeasy mini kit with QIAshredder and cDNA was synthesized using a Bioline Tetro … WebMar 9, 2016 · I have used Bioline's Tetro cDNA synthesis kit for synthesis of cDNA using random hexamers as primer with E. coli RNA samples to measure gene expression by quantitative real time PCR. The …
WebEpiNext cTIP (CUT&Tag In-Place)-Sequencing Kit Description: The EpiNext™ cTIP (CUT&Tag In-Place)-Sequencing Kit is a complete set of reagents required for carrying out a ... Quantity: 12 reactions/ 24 reactions Applications: Immunoprecipitation, Library Construction Supplier Page Sign In or Register to view pricing Compare Product + See … WebBioline Pricing 2024 - Read online for free. Scribd is the world's largest social reading and publishing site. ... 6,250,000 BIO-65042 Tetro cDNA Synthesis Kit 30 Reactions 2,100,000 BIO-65043 Tetro cDNA Synthesis Kit 100 Reactions 3,650,000 BIO-65048 MyTaq One-Step RT-PCR Kit 25 Reactions 1,250,000 BIO-65049 MyTaq One-Step RT-PCR Kit 100 ...
WebMar 31, 2024 · A Tetro cDNA synthesis kit (catalogue no. BIO-65043, Bioline, London, UK) was used to synthesise cDNA using random primers, while following the manufacturer’s instructions. Two genes from each condition (YPD-28 °C, YPD-16 °C, and YP + glycerol-16 °C) showing significant transcriptional changes in RNAseq dataset were picked for further ...
WebAug 5, 2016 · Bioline's Tetro cDNA synthesis kit was used to synthesis first strand cDNA using Salmonella enterica total RNA to quantify gene expression. A total of 1 microgram RNA was used and at the end of the … flshinesWeb…the SensiFAST cDNA Synthesis Kit and SensiFAST Real-Time PCR Kits, or the SensiFAST One-Step Real-Time RT-PCR Kits. Additionally, the ISOLATE II RNA Plant Kit can be used to purify samples prior to RT-PCR amplification using the Tetro cDNA Synthesis Kit and any enzyme from the Bioline PCR portfolio,… green day - international superhitsWebApr 13, 2024 · Lipofectamine 2000, Tetro cDNA synthesis kit and QuickExtract DNA extraction were ordered from Invitrogen (USA), Bioline (Germany), and Lucigen (USA), respectively. Primary and secondary antibodies, and fluorescence-tagged secondary antibodies were purchased from Santa Cruz Biotechnology (USA). Midorigreen was … fl sheriff\u0027s youth ranchWebJan 6, 2024 · Subsequently, the Tetro cDNA Synthesis Kit was used to convert all RNA samples to cDNA in a 96-well plate using T 100™ Thermal Cycler (Bio-Rad). The qPCR reactions were started by adding 10 ng cDNA to 5 μl SensiFAST™ SYBR and 500 nM forward and reverse primers (Table 3) in a 384-well plate and in duplicates. green day iphone wallpaperWebThe product portfolio comprises more than 300 reagents and kits, many of them proprietary, for molecular biology and cell analysis. Bioline is an ISO 13485 certified company and one of the world’s few manufacturers of … fl sheriffs youth ranchesWebEach reaction contained 1 μg of RNA diluted in a volume of 11 µL, to which we added 9 µL of cDNA synthesis mix (Tetro cDNA synthesis kit; Bioline, Redfern, NSW, Australia). Samples were incubated at 45 °C for 40 min followed by 85 °C for 5 min. flshield.comWebTetro™ cDNA Synthesis Kit Shipping: On Dry/Blue Ice Catalog numbers BIO-65042: 30 reactions Batch No.: See vial Quality Control: BIO-65043: 100 reactions Storage and … green day induction to hall of fame