Clonogenic capacity

Author: lljp

August undefined, 2024

WebJan 2, 2024 · Clonogenic assays. The clonogenicity of cells is commonly measured using a clonogenic assay, or colony formation assay that measure the ability of cells to divide … WebMar 6, 2024 · (F) The reduced clonogenic capacity of Capan-1cell line after specific siRNA knockdown of NAT10. (G) The IC50 of gemcitabine in Capan-1cells after specific siRNA knockdown of NAT10. The data are the mean ± SEM. *P<0.05, **P<0.01.

Functional features of cancer stem cells in melanoma cell lines

WebJul 22, 2009 · We have previously shown that reduced Erk phosphorylation as a consequence of the U0126 inhibitor, correlates with reduced clonogenic and proliferative capacity of K562 cells. CCN3 may compromise cell survival by reducing Erk phosphorylation causing decreased expression of pro-survival factors such as BCL-2, … WebMar 15, 2004 · To examine the clonogenic capacity of these subpopulations, we isolated CD138 + and CD138-cells from both cell lines by FACS and examined each subpopulation for colony formation in methylcellulose. To examine self-renewal potential, colonies were scored weekly, collected, and then washed and serially replated. convert string to offsetdatetime in java

Clonogenic capacity and ex vivo expansion potential of umbilical …

WebAug 22, 2024 · It showed that tanshinone IIA inhibited radiation-induced cardiomyocyte injury while enhancing the proliferative capacity of the cells. When further administration of p38 MAPK agonist treatment, it was found that the cell clonogenic rate decreased significantly ( ), and the LDH level also increased significantly ( ), suggesting that p38 … WebOct 25, 2024 · The clonogenic assay measures the capacity of single cells to form colonies in vitro. It is widely used to identify and quantify self-renewing mammalian cells … WebMay 31, 2024 · In addition, knockdown of p32 significantly decreased clonogenic capacity and in vivo tumorigenesis in a xenograft mice model. Altogether, our results demonstrate … convert string to predicate in java

Clonogenic assay of cells in vitro Nature Protocols

Treatment with G-CSF reduces acute myeloid leukemia blast …

WebDec 5, 2014 · ECFCs are a synthetic representation of vasculogenic capacity, which varies depending on input cell source. We chose to use this laboratory assay in parallel with a cardiovascular cell therapy trial because the target organ for repair—the heart—suffered from ischemia and infarction. ... CD11b, and clonogenic capacity as biomarkers for ... WebOct 29, 2024 · The clonogenic assay is a versatile and frequently used tool to quantify reproductive cell survival in vitro. Current state-of-the-art analysis relies on plating efficiency-based calculations which assume a linear correlation between the number of cells seeded and the number of colonies counted. The present study was designed to … false or true pythonWebCFU-E (Colony forming unit-erythroid): Clonogenic progenitors that produce only one or two clusters with each cluster containing from 8 to approximately 100 hemoglobinized erythroblasts. It represents the more mature erythroid progenitors that have less proliferative capacity. ... These are primitive erythroid progenitors that have high ... false or incorrect

"WebSince several programs throughout the world are currently evaluating the feasibility of large-scale UCB banking for unrelated transplants, it was the aim of this study to evaluate … " - Clonogenic capacity

Clonogenic capacity

Molecules Free Full-Text rhTPO Ameliorates Radiation-Induced …

WebSep 12, 2024 · Clonogenic capacity depends upon cell type because many cells proliferate well in the culture but takes weeks to form … WebFeb 1, 2024 · As shown in Fig. 8 A, d-limonene significantly reduced the clonogenic capacity of SH-SY5Y cells. Moreover, inhibition of clonogenic survival, evaluated after …

Did you know?

WebFeb 1, 2013 · Colony-forming capacity (CFC) was expressed as the number of spheres at least 50 μm in diameter, generated by the 1 × 10 3 cells seeded before. Fold change in … WebJun 10, 2024 · Furthermore, coincubation with TLSi restored cyclin E1–induced ssDNA gaps and reduced the clonogenic capacity of the pro-TLS cells, but had no effect on colonies without any oncogene induction (Fig. 3, C and F, and fig. S3B). Collectively, these findings indicate that TLS polymerases buffer oncogene-induced stress to facilitate continuous ...

WebMay 16, 2024 · The remarkable tumor suppressor capacity of PCNP in vivo was confirmed by IHC analysis for proliferation (Ki67), associated angiogenesis (CD31), apoptosis (cleaved caspase-3), cell cycle (p21), autophagy (Beclin-1) of tumor sections from tumors harvested from control, Mock, PCNP, sh-sub, and sh-PCNP group mice. PCNP may act as a … WebThe clonogenic assay is an in vitro cell survival assay that evaluates all modalities of cell death based on the ability of a single cell to grow into a colony. The colony is defined to …

WebDec 7, 2024 · Culture of CD34 + cells with HIF-MSC increases their clonogenic capacity in vitro. Regarding the evaluation of in vitro functional effects induced after co-culture, we observed that the capacity of CD34 + cells to form CFU-GM colonies was significantly higher when they were co-cultured with HIF-MSC in comparison with MSC WT (p = 0.048 WebFeb 1, 2024 · As shown in Fig. 8 A, d-limonene significantly reduced the clonogenic capacity of SH-SY5Y cells. Moreover, inhibition of clonogenic survival, evaluated after 12 days incubation (Fig. 8 A), occurs in a concentration-dependent manner exactly matching that promoting early LC3 lipidation (Russo et al., 2014 and Fig. 5 A). These observations …

WebA clonogenic assay, also known as a colony formation assay is an in vitro cell survival assay. It assesses the ability of single cells to survive and reproduce to form colonies1. This assay was first described in the 1950s, …

WebThe clonogenic assay is an in vitro cell survival assay that evaluates all modalities of cell death based on the ability of a single cell to grow into a colony. The colony is defined to … false outWebAug 4, 2024 · We uncovered the emergence of blood progenitor and erythroid-like cell populations in late gastruloids and showed the multipotent clonogenic capacity of these cells, both in vitro and after ... convert string to number spssWebclonogenic: ( klō'nō-jen'ik ), Arising from or consisting of a clone. convert string to num in jsWebApr 8, 2024 · Clonogenic survival assay Single-cell suspensions were inoculated into six-well plates at densities of 400–12,000 cells per well. After 12 h, cells were irradiated with six MV X-rays at gradient doses of 0, 2, 4, 6, and 8 Gy using an X-ray linear accelerator (Clinac 2100EX, Varian Medical Systems) with a 300 cGy/min dose rate and 100 cm ... convert string to outputstream javaWebThe clonogenic assay is a well-established in vitro method for testing the survival and proliferative capability of cells. ... radiation. However, this approach can also characterize … convert string to operator javascriptWebAug 1, 2024 · The clonogenic capacity of HSPCs was enhanced in preterm cord blood. Using univariate analysis, the number and clonogenic potential of circulating UCB HSPCs was influenced by gestational age, birth weight and maternal age. Multivariate analysis showed that main factors that significantly influenced the HSPC count were maternal … false outlet backroomsWebThe clonogenic assay measures the capacity of single cells to form colonies in vitro. It is widely used to identify and quantify self-renewing mammalian cells derived from in vitro cultures as well as from ex vivo tissue preparations of different origins. Varying research questions and the heterogeneous growth requirements of individual cell ... convert string to privatekey java