WebAppressorium turgor was estimated using an adaptation of the incipient cytorrhysis (cell collapse) assay (Howard et al., 1991; Dixon et al., 1999). Appressoria were allowed to form on plastic coverslips incubated in a humid chamber for 24 or 48 h, and the surface water was removed carefully with a pipette and replaced with an equal volume of ... WebIn an incipient cytorrhysis (cell collapse) assay using a 1–4 molar concentration of glycerol solution, appressoria of the ΔMomip11 mutant showed increased collapse rates in …
Activation of Mst11 and Feedback Inhibition of Germ Tube …
WebDec 22, 2006 · We therefore estimated the appressorial turgor through the incipient cytorrhysis assays (Howard et al., ... that the Abc3-GFP protein was highly expressed and concentrated in the appressorial plasma membrane right from the incipient stage onwards . However, at a later stage (17 h after germination) during ... WebSep 1, 1990 · The diameter of the largest pores in the hyphal cell wall ofAchlya bisexualis was estimated by scoring cells for plasmolysis and cytorrhysis in polyethylene glycol (PEG) solutions covering a range of different molecular weights. The cell wall was freely permeable to PEGs with an average molecular weight of ≤600, but excluded PEG-1500 and ... grand total of building material 9 letters
The glyoxylate cycle is required for temporal regulation of …
WebOct 18, 2024 · Panel (A) shows incipient cytorrhysis assays carried out to measure appressorium turgor for the ΔMoOeif3k, ΔMoOeif3k_Com, and wild-type strains inoculated on appressorium-inducing hydrophobic coverslips for 8 h. The appressoria were treated with different concentrations (1, 2, 3, and 4 M) of glycerol solutions. WebApr 11, 2014 · We applied incipient cytorrhysis to investigate the appressorial turgor pressure of P. pachyrhizi. Mature appressoria were … WebApr 6, 2007 · Appressorial turgor was estimated using incipient cytorrhysis (cell collapse) assays ( 6 ). Conidia in 20-μl droplets (1 × 10 6 conidia/ml) were induced to form … chinese rugs blue