Thermo t7体外转录试剂盒
Webb产品介绍. T7体外转录试剂盒 人线粒体基因组的特点: 1 、人线粒体基因组为 16 , 569bp 的双链闭环分子,一条链为重链 (H 链) ,一条链为轻链 (L 链) ,两条链均有编码功能, … WebbExhibits greater specific activity than WT-enzyme at 37-50ºC. Thermo T7 RNA polymerase is a genetically modified T7 RNA polymerase exhibiting increased thermal stability. The optimum reaction temperature of this enzyme is around 50ºC. The half-life of the enzyme at 50ºC is approximately 85 min.
Thermo t7体外转录试剂盒
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Webb本试剂盒能以携带有T7 Promoter (TAATACGACTCACTATAGGG)序列的线性化质粒、PCR产物或合成的DNA片段等为模板,体外快速转录合成mRNA、lncRNA、shRNA等多种类型 … WebbThermo T7 RNA polymerase. Thermo T7 RNA polymerase is a genetically modified T7 RNA polymerase exhibiting increased thermal stability compared with other RNA polymerases. The optimum reaction temperature of this enzyme is approximately 50°C. The half-life of the enzyme at 50°C is approximately 85 min.
WebbThermo Scientific TranscriptAid T7 High Yield Transcription Kit contains reagents for 50 reactions of 20 µL. Each reaction yields up to 200 µg RNA from 1 µg of template in 2 hours. The reaction can be scaled up to … WebbT7 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride … The MEGAscript™ T7 Kit is an ultra-high yield in vitro transcription kit. The high … The MEGAscript™ T7 Kit is an ultra-high yield in vitro transcription kit. The high …
Webb二是需要转录的dna序列的上游必须有t7启动子。如果模板是pcr产物,则 可以在设计引物时将t7启动子序列(5’taa tac gac tca cta tag gg 3’)加上。如果是将dna片段克隆到载体上,则需要选择有t7启动子的载体, 并且克隆位点必须位于t7启动子下游。 Webb本发明公开了一种柑橘黄脉病毒微滴数字pcr定量检测试剂盒,包括总rna提取试剂,引物cp7、cp8和cp9,t7体外转录试剂盒,qx200 ...
Webb用最低浓度的标记核苷酸进行转录反应,可能由于核苷酸浓度不足而产生过早终止的转录组。. 增加限制性核苷酸的浓度通常会提高全长转录组的产量。. 降低反应的孵育温度. 通常,转录反应在室温或 37°C 下进行。. 将温度降低至约 16°C 或甚至 4°C 有时可以改善 ...
http://bjbalb.com/html/Nucleic-Acid-Amplification/BTN91106.html ride shiny and chromeWebbCatalog number: EP0111. Thermo Scientific Bacteriophage T7 RNA polymerase is a DNA-dependent RNA polymerase with strict specificity for its respective double-stranded promoters. It catalyzes the 5'→3' … ride shippingWebb体外转录系统性能比较. Promega RiboMAX™ Systems 可生成的高质量RNA转录本可直接用于体外或体内翻译。. 客户可以修饰DNA序列,将DNA快速地转录为RNA,再通过RNA的翻译确定DNA修饰所造成的影响。. 同样,编码目标蛋白的DNA序列也可被快速转录为mRNA,然后包装进选定的 ... ride ship agencyWebbThe Thermo Scientific™ TranscriptAid™ T7 High Yield Transcription Kit is designed for high yield in vitro transcription from DNA templates containing T7 RNA Polymerase promoter. The kit contains reagents for 50 reactions of 20 µL. Depending on transcript length each reaction yields approximately 150 µg RNA ride share uberWebbSilencer siRNA 构建试剂盒所包含的体外转录方法使用 T7 RNA 聚合酶生成 siRNA 单链。 反应模板是由两个可编码目标 siRNA 链的廉价 DNA 寡核苷酸生成。 设计这些寡核苷酸时 … ride shirtWebbAmbion™ MAXIscript™ SP6/T7 体外 转录试剂盒在仅10分钟内合成 RNA 探针,以便用于核糖核酸酶保护测定、 原位 杂交和印迹杂交。 各试剂盒包含足够进行50次各酶反应的试 … ride show hallmarkWebb本试剂盒是基于sp6 rna聚合酶的rna体外转录试剂盒,它利用含有sp6启动子的模版dna,以ntp为底物,从sp6启动子下游开始合成与模版dna中一条链互补的rna,简单快速获得大量的rna分子。 ride short interest fintel